NEFL antibody

Catalog No. ABIN6654562

The Rabbit Monoclonal anti-NEFL antibody has been validated for IHC (p). It is suitable to detect NEFL in samples from Human.

Quick Overview for NEFL antibody (ABIN6654562)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: NEFL-2983R

Product Details anti-NEFL Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the recombinant Neurofilament antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Neurofilament antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: Neurofilament (-Light) / NF-L / NEFL

Background: This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.