CRYAB antibody

Catalog No. ABIN6654631

The Mouse Monoclonal anti-CRYAB antibody has been validated for WB and IHC (p). It is suitable to detect CRYAB in samples from Human and Rat.

Quick Overview for CRYAB antibody (ABIN6654631)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CPTC-CRYAB-1

Product Details anti-CRYAB Antibody

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: A full length recombinant human protein was used as the immunogen for the CRYAB antibody.

Isotype: IgG2c

Application Details

Application Notes: Optimal dilution of the CRYAB antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CRYAB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: CRYAB

Background: Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. Crystallins are divided into alpha, beta and gamma families, and the beta- and gamma-crystallins also compose a superfamily. Crystallins usually contain seven distinct protein regions, including four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha-crystallins consist of three gene products, alpha-A-, alpha-B- and alpha-C-crystallin, which are members of the small heat shock protein family (HSP 20). Alpha-crystallins act as molecular chaperones by holding denatured proteins in large soluble aggregates. However, unlike other molecular chaperones, alpha-crystallins do not renature these proteins. Expression of alpha-A-crystallin is restricted to the lens and defects of this gene cause the development of autosomal dominant congenital cataracts (ADCC). The human alpha-B-crystallin gene product is expressed in many tissues, including lens, heart and skeletal muscle. Elevated expression of alpha-B-crystallin is associated with many neurological diseases, and a missense mutation in this gene has co-segregated in a family with a Desmin-related myopathy.