Nkx2-2 antibody

Catalog No. ABIN6654697

The Mouse Monoclonal anti-Nkx2-2 antibody has been validated for IF, FACS and IHC (p). It is suitable to detect Nkx2-2 in samples from Human, Mouse, Rat and Chicken.

Quick Overview for Nkx2-2 antibody (ABIN6654697)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RNX2-294

Product Details anti-Nkx2-2 Antibody

Purification: Purified

Purity: Protein G affinity chromatography

Immunogen: Full length human recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant NKX2.2 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

Pathways: Dopaminergic Neurogenesis