Nkx2-2 antibody (N-Term)

Catalog No. ABIN2779575

There is 1 publication for this product available. The Rabbit Polyclonal anti-Nkx2-2 antibody is suitable to detect Nkx2-2 in samples from Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse and Sheep. It has been validated for IHC and WB.

Quick Overview for Nkx2-2 antibody (N-Term) (ABIN2779575)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Guinea Pig, Horse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Product Details anti-Nkx2-2 Antibody

Binding Specificity: N-Term

Purpose: NKX2-2 Antibody - N-terminal region

Sequence: MSLTNTKTGF SVKDILDLPD TNDEEGSVAE GPEEENEGPE PAKRAGPLGQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 92%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against NKX2-2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NKX2-2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32338-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Nkx2-2 antibody (ABIN2779575)

Owen, Kowalewski, Lessnick: "EWS/FLI mediates transcriptional repression via NKX2.2 during oncogenic transformation in Ewing's sarcoma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1965, (2008) (PubMed).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2-2

Background:

Background Information: Nkx2-2 contains 1 homeobox DNA-binding domain which is essential for interaction with OLIG2. Nkx2-2 may be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.

Gene Name: NK2 homeobox 2

Alternative Symbols: NKX2B, NKX2.2

Protein Name: Homeobox protein Nkx-2.2

Molecular Weight: 30kDa

Gene ID: 4821

NCBI Accession: NP_002500

UniProt: O95096

Pathways: Dopaminergic Neurogenesis