pan Keratin antibody

Catalog No. ABIN6654982

The Rabbit Monoclonal anti-pan Keratin antibody has been validated for IF, FACS, WB and IHC (p). It is suitable to detect pan Keratin in samples from Human, Rat, Mouse, Cow, Monkey, Pig, Frog, Guinea Pig and Goat.

Quick Overview for pan Keratin antibody (ABIN6654982)

Target: pan Keratin (panKRT)

Reactivity: Human, Rat, Mouse, Cow, Monkey, Pig, Frog, Guinea Pig, Goat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: KRT-1877R

Product Details anti-pan Keratin Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: A Keratin-enriched preparation from cultured human epithelial cells was used as the immunogen for the recombinant Multi Cytokeratin antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Multi Cytokeratin antibody should be determined by the researcher.

1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Multi Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Multi Cytokeratin

Background: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This mAb recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum mAb which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.