HSPH1 antibody

Catalog No. ABIN6655020

Supplier Product No.: 600-401-f52

This Rabbit Polyclonal antibody specifically detects HSPH1 in WB, IHC, IF and FM. It exhibits reactivity toward Armenian Hamster.

Quick Overview for HSPH1 antibody (ABIN6655020)

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Reactivity: Armenian Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-HSPH1 Antibody

Supplier Product No.: 600-401-f52

Supplier: Rockland

Purpose: Hsp110 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp110 from Human, Monkey, Hamster, Rat, Mouse, Cow, Sheep, and Yeast based on 100 % homology with the immunizing sequence.

Purification: Anti-Hsp110 Antibody was purified by affinity chromatography.

Sterility: Sterile filtered

Immunogen: Hsp110 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against a synthetic peptide derived from the sequence of hamster Hsp110.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-Hsp110 Antibody is tested for use in WB, IHC, and IF/ICC. Expect a band approximately ~110kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HSPH1

Target: HSPH1 (Heat Shock 105kDa/110kDa Protein 1 (HSPH1))

Alternative Name: HSPH1

Background:

Synonyms: Heat shock 105 kDa alpha, heat shock 105 kDa beta, Hsp105, Hsp105A, Hsp105B, Hsp110, HSPH1, NY CO 25, Heat shock 110 kDa protein

Background: Hsp110 belongs to a family of large stress proteins known as the Hsp110/SSE Family. The proteins in this family are the most distantly known relatives of the well studied Hsp70 family. They share 30-33 % amino acid identity, mostly in the conserved ATP-binding domain. Hsp110 cooperates with Hsp70 in protein folding in the eukaryotic cytosol. In mammals, Hsp110 is constitutively expressed, but exhibits particularly high levels in the brain. Both Hsp70 and Hsp110 are elevated after cerebral ischemia. Recent studies demonstrate that the protective effects of Hsp110 deficiency in cerebral ischemia may partly be mediated by an increase in the chaperone activity of Hsp70. Studies also suggest that Hsp110 can be used in heat shock protein-based cancer immunotherapy.

Gene Name: HSPH1

Gene ID: 100689462

NCBI Accession: NP_001233649

UniProt: Q60446