Anthrax Protective Antigen antibody (Internal Region)

Catalog No. ABIN6655029

Supplier Product No.: 100-401-h55

This Rabbit Polyclonal antibody specifically detects Anthrax Protective Antigen in WB. It exhibits reactivity toward Bacillus anthracis.

Quick Overview for Anthrax Protective Antigen antibody (Internal Region) (ABIN6655029)

Target: Anthrax Protective Antigen (PA)

Reactivity: Bacillus anthracis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Anthrax Protective Antigen antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Anthrax Protective Antigen Antibody

Binding Specificity: Internal Region

Supplier Product No.: 100-401-h55

Supplier: Rockland

Purpose: Anthrax Protective Antigen Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Anthrax PA from multiple species based on 100 % homology with the immunizing sequence.

Purification: Anti-Anthrax Protective Antigen Antibody is sera.

Immunogen: Anthrax Protective Antigen Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the internal region of the Anthrax PA protein.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-Anthrax PA (Protective Antigen) Antibody has been tested in WB. Expect a band approximately 83kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Anthrax Protective Antigen

Target: Anthrax Protective Antigen (PA)

Alternative Name: Anthrax Protective Antigen

Background:

Synonyms: pag, Anthrax Protective Antigen, Anthrax toxins translocating protein, PA-83, Protective antigen PA-20, PA20, Protective antigen PA-63, PA63

Background: Anthrax PA (Protective Antigen) and Anthrax LF (Lethal Factor), along with Anthrax EF (Edema Factor), are toxins produced by Bacillus anthracis. All three of the proteins must be present for toxic entry into cells. Anthrax PA is secreted from Bacillus anthracis as an 83 kD (PA83) protein which is cleaved by furin protease, releasing a 20 kD fragment. The 63 kD portion (PA63) of the cleavage product forms a heptamer, which binds the toxins LF and EF. This complex enters via receptor-mediated endocytosis where LF and EF exert their toxic effects. Anthrax PA is ideal for researchers interested in Neurobiology research.

Gene Name: pagA

Gene ID: 1158723

NCBI Accession: NP_052806

UniProt: P13423