GRP94 antibody

Catalog No. ABIN6655034

Supplier Product No.: 200-501-f39

This anti-GRP94 antibody is a Rat Monoclonal antibody detecting GRP94 in WB, FACS, IF and IP. Suitable for Chicken.

Quick Overview for GRP94 antibody (ABIN6655034)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Chicken

Host: Rat

Clonality: Monoclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 9G10

Product Details anti-GRP94 Antibody

Supplier Product No.: 200-501-f39

Supplier: Rockland

Purpose: GRP94 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with GRP94 from Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea pig, Hamster, Horse, Monkey, Pig, Rabbit, Sheep, Xenopus sources based on 100 % homology with the immunizing sequence.

Purification: Anti-GRP94 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Grp94 Antibody was produced in rat by repeated immunizations with purified Grp94 isolated from chicken oviducts.

Isotype: IgG2a

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: User Optimized

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-Grp94 Antibody is tested for WB, IF, IP, and Flow cytometry. Expect a band approximately 98kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. This monoclonal antibody reacts with purified Grp94. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: GRP94

Background:

Synonyms: HSP90B1, GP96, TRA1, ECGP, Endoplasmin, 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog

Background: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion. Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90. Both Hsp90 and Grp94 are calcium binding proteins. Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity. Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER

Gene Name: HSP90B1

Gene ID: 374163

NCBI Accession: NP_989620

UniProt: P08110

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades