Rho-related GTP-binding protein antibody

Catalog No. ABIN6655050

Supplier Product No.: 200-301-g37

This Mouse Monoclonal antibody specifically detects Rho-related GTP-binding protein in ELISA, WB, IHC, IP, IF and FM. It exhibits reactivity toward Cow.

Quick Overview for Rho-related GTP-binding protein antibody (ABIN6655050)

Target: Rho-related GTP-binding protein (RhO (pan))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Rho-related GTP-binding protein antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 1D4

Product Details anti-Rho-related GTP-binding protein Antibody

Supplier Product No.: 200-301-g37

Supplier: Rockland

Purpose: Rhodopsin Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Rhodopsin from all vertebrates based on 100 % homology with the immunizing sequence.

Purification: Anti-Rhodopsin Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Rhodopsin Antibody was produced in mice by repeated immunizations raised against bovine Rhodopsin.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-Rhodopsin Antibody is tested for use in IF microscopy, IHC, IP, WB, and ELISA. Expect a band approximately ~40kDa corresponding to specific lysates. Binds specifically to the N-terminus of Rhodopsin. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Rho-related GTP-binding protein

Target: Rho-related GTP-binding protein (RhO (pan))

Alternative Name: RHO

Background:

Synonyms: OPN2 Antibody, opsd Antibody, opsin 2 Antibody, opsin 2 rod pigment Antibody, opsin2 Antibody, RHO Antibody, RP4 Antibody, MGC138309 Antibody, Retinitis Pigmentosa 4 Antibody, Rhodopsin Antibody

Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption. In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin. Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder.

Gene Name: RHO

Gene ID: 509933

NCBI Accession: NP_001014890

UniProt: P02699