GFAP antibody

Catalog No. ABIN6655052

Supplier Product No.: 100-401-d60

This anti-GFAP antibody is a Rabbit Polyclonal antibody detecting GFAP in WB, IHC and FM. Suitable for Cow. This Primary Antibody has been cited in 1 publication.

Quick Overview for GFAP antibody (ABIN6655052)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Product Details anti-GFAP Antibody

Supplier Product No.: 100-401-d60

Supplier: Rockland

Purpose: Glial Fibrillary Acidic Protein (GFAP) Antibody

Cross-Reactivity (Details): The antibody is directed against bovine glial fibrillary acidic proteins.

Purification: Anti-GFAP antibody was prepared from monospecifc neat serum.

Immunogen: Anti-GFAP Antibody was produced in rabbit by repeated immunizations with recombinant and purified bovine glial fibrillary acidic protein.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Glial Fibrillary Acidic Protein (Rabbit) antibody has been tested by western blot, ICC, and IHC and is suitable for use in IF. Anti-Glial Fibrillary Acidic Protein antibodies are specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFAP antibody (ABIN6655052)

Qin, Buckley, Li, Liu, Fox, Meares, Yu, Yan, Harms, Li, Standaert, Benveniste: "Inhibition of the JAK/STAT Pathway Protects Against α-Synuclein-Induced Neuroinflammation and Dopaminergic Neurodegeneration." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 18, pp. 5144-59, (2017) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Background:

Synonyms: gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, cb345.

Background: Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques. It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers. Therefore, GFAP antibody is ideal for investigators involved in neuropathologic diseases and more generally in Neuroscience.

Gene Name: GFAP

Gene ID: 281189

UniProt: Q28115