Spectrin, Alpha, beta antibody

Catalog No. ABIN6655089

Supplier Product No.: 200-301-p46

This Mouse Monoclonal antibody specifically detects Spectrin, Alpha, beta in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Spectrin, Alpha, beta antibody (ABIN6655089)

Target: Spectrin, Alpha, beta

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB), ELISA

Clone: SB-SP1

Product Details anti-Spectrin, Alpha, beta Antibody

Supplier Product No.: 200-301-p46

Supplier: Rockland

Purpose: Spectrin (alpha and beta) Antibody

Cross-Reactivity (Details): This product reacts with human.

Purification: Anti-Spectrin antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Spectrin(alpha and beta) antibody was produced in mice by repeated immunizations with human erythrocyte spectrin.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:40,000

Western_Blot_Dilution: 2-4 μg/mL

Comment:

Anti-Spectrin is tested for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~246-280 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Spectrin, Alpha, beta

Target: Spectrin, Alpha, beta

Alternative Name: Spectrin (alpha and beta)

Background:

Synonyms: EL2, HPP, HS3, SPH3, SPTA, EL3, HS2, SPH2, HSPTB1, Erythroid alpha-spectrin

Background: Spectrin, the predominant component of the membrane skeleton of the red cell, is essential in determining the properties of the membrane including its shape and deformability. It consists of 2 nonidentical subunits, alpha and beta. Spectrin is present in the red cell membrane in a tetrameric or possibly higher polymeric form through head-to-head self-association of heterodimers that are linked by actin polymers and protein 4.1 to form a 2-dimensional network. Non-erythroid spectrin gene is mapped to human chromosome 2. Spectrin mutations cause spinocerebellar ataxia type 5. This antibody is suitable for researchers interested in cytoskeleton and transcriptoion factor research.

Gene Name: SPTA1, SPTB

Gene ID: 6708

NCBI Accession: NP_003117

UniProt: P02549