CALB1 antibody

Catalog No. ABIN6655092

Supplier Product No.: 200-301-p22

This Mouse Monoclonal antibody specifically detects CALB1 in WB, IHC, ELISA, IP and IF. It exhibits reactivity toward Cow.

Quick Overview for CALB1 antibody (ABIN6655092)

Target: CALB1 (Calbindin (CALB1))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This CALB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: CB-955

Product Details anti-CALB1 Antibody

Supplier Product No.: 200-301-p22

Supplier: Rockland

Purpose: Calbindin-D Antibody

Cross-Reactivity (Details): This product reacts with human, mouse, bovine, and rat.

Purification: Anti-Calbindin-D antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Calbindin-D antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to bovine kidney calbindin-D.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1 μg/mL

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1-2 μg/mL

Western_Blot_Dilution: 0.5-1 μg/mL

Comment:

Anti-Calbindin-D is tested for Immunofluorescence, Immunohistochemistry -P and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~30 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CALB1

Target: CALB1 (Calbindin (CALB1))

Alternative Name: CALB1

Background:

Synonyms: CALB, D-28K, CALBINDIN, 28-KD, Calbindin D28, Vitamin D-dependent calcium-binding protein, avian-type

Background: The human calbindin D28k (CALB1) is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. Parmentier et al. (1991) mapped the CALB1 gene to 8q21.3-q22.1 by in situ hybridization. This antibody is suitable for researchers interested in transcription factors, nuclear receptors, cancer research, and nutritional and metobolic diseases.

Gene Name: CALB1

Gene ID: 793

NCBI Accession: NP_004920

UniProt: P05937