CD69 antibody (APC)

Catalog No. ABIN6655125

Supplier Product No.: 200-326-n73

This Mouse Monoclonal antibody specifically detects CD69 in FACS, IHC and FM. It exhibits reactivity toward Human.

Quick Overview for CD69 antibody (APC) (ABIN6655125)

Target: CD69

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD69 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Clone: FN50

Product Details anti-CD69 Antibody (APC)

Supplier Product No.: 200-326-n73

Supplier: Rockland

Purpose: CD69 Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD69.

Purification: Allophycocyanin conjugated CD69 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD69.

Sterility: Sterile filtered

Immunogen: Anti-CD69 Antibody (Monoclonal) was produced by repeated immunizations with CD69 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD69 is tested for Flow Cytometry and is useful in Immunohistochemistry and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD69

Target: CD69

Alternative Name: CD69

Background:

Synonyms: Early activation antigen CD69, Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69, CLEC2C

Background: CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Gene Name: CD69

Gene ID: 969

NCBI Accession: NP_001772

UniProt: Q07108