GREM1 antibody

Catalog No. ABIN6655147

Supplier Product No.: 200-401-h77

The Rabbit Polyclonal anti-GREM1 antibody has been validated for WB and IHC. It is suitable to detect GREM1 in samples from Human.

Quick Overview for GREM1 antibody (ABIN6655147)

Target: GREM1 (Gremlin 1 (GREM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GREM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GREM1 Antibody

Supplier Product No.: 200-401-h77

Supplier: Rockland

Purpose: Gremlin Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Gremlin from Human, Rat and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-Gremlin Antibody was purified by Protein G chromatography.

Immunogen: Gremlin Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the internal amino acid sequences within the Gremlin-1 human protein.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 2.5-10 μg/mL (paraffin)

Western_Blot_Dilution: 1:500-1:1000

Comment:

Anti-Gremlin Antibody is tested for use in IHC (cryosections) and IHC-P. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GREM1

Target: GREM1 (Gremlin 1 (GREM1))

Alternative Name: Gremlin

Background:

Synonyms: CKTSF1B1, DAND2, DRM, Gremlin-1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2

Background: Anti-Gremlin Antibody detects human Gremlin. In Xenopus, Gremlin belongs to a novel gene family that act as BMP antagonists in embryonic explants. The human homolog of Gremlin (CKTSF1B1) is localized on chromosome 15q13--> q15. DRM-specific mRNA is expressed in different tissues, including brain, ovary, intestine and colon. It has been suggested that down-regulation of Gremlin is associated with tumor progression, and support the hypothesis that Gremlin may play an important role during both neuroembryological development and carcinogenesis. Anti-Gremlin Antibody is ideal for investigators involved in NFkappaB, signaling, and cell cycle protein research.

Gene Name: GREM1

Gene ID: 26585

NCBI Accession: NP_001178252

UniProt: O60565

Pathways: Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location