CD3 epsilon antibody (APC)

Catalog No. ABIN6655175

Supplier Product No.: 200-326-n12

The APC-conjugated Mouse Monoclonal anti-CD3 epsilon antibody (Clone HIT3a) (ABIN6655175) specifically detects CD3 epsilon in FACS, IHC and IP. The antibody is reactive with Human samples.

Quick Overview for CD3 epsilon antibody (APC) (ABIN6655175)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: HIT3a

Product Details anti-CD3 epsilon Antibody (APC)

Supplier Product No.: 200-326-n12

Supplier: Rockland

Purpose: CD3 Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD3.

Purification: Allophycocyanin conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3.

Sterility: Sterile filtered

Immunogen: Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Comment:

Anti-CD3 is tested in Flow Cytometry and useful for Immunoprecipitation, Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Background: CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Gene Name: CD3E

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway