CD3 epsilon antibody (FITC)

Catalog No. ABIN6655177

Supplier Product No.: 200-302-n12

The Mouse Monoclonal anti-CD3 epsilon antibody is suitable to detect CD3 epsilon in samples from Human. It has been validated for FACS, IHC and IP.

Quick Overview for CD3 epsilon antibody (FITC) (ABIN6655177)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: HIT3a

Product Details anti-CD3 epsilon Antibody (FITC)

Supplier Product No.: 200-302-n12

Supplier: Rockland

Purpose: CD3 Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD3.

Purification: Fluorescein conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3.

Sterility: Sterile filtered

Immunogen: Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.

Isotype: IgG2a kappa

Labeling Ratio: 4-6

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Comment:

Anti-CD3 has been tested in Flow Cytometry and is useful for Immunoprecipitation, and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Background: CD3 epsilon is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3e, one CD3 gamma, one CD3 delta, one CD3 zeta (CD247), and a T-cell receptor ( alpha/beta or gamma/delta) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Gene Name: CD3E

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway