CD3 epsilon antibody (PE)

Catalog No. ABIN6655180

Supplier Product No.: 200-308-n12

The Mouse Monoclonal anti-CD3 epsilon antibody is suitable to detect CD3 epsilon in samples from Human. It has been validated for FACS.

Quick Overview for CD3 epsilon antibody (PE) (ABIN6655180)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: HIT3a

Product Details anti-CD3 epsilon Antibody (PE)

Supplier Product No.: 200-308-n12

Supplier: Rockland

Purpose: CD3 Phycoerythrin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD3.

Purification: Phycoerythrin conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3.

Sterility: Sterile filtered

Immunogen: Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.

Isotype: IgG2a kappa

Labeling Ratio: 1-2

Application Details

Application Notes: Optional[Neutralization_Dilution]: 5 uL/test/1x10e5 to 1x10e8 cells

Comment:

Anti-CD3 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3E

Background:

Synonyms: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e

Background: The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The Hit3a antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of Hit3a antibody to CD3e can induce cell activation. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3.

Gene Name: CD3E

Gene ID: 916

NCBI Accession: NP_000724

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway