CD8 alpha antibody (FITC)

Catalog No. ABIN6655185

Supplier Product No.: 200-302-m64

This Mouse Monoclonal antibody specifically detects CD8 alpha in FACS. It exhibits reactivity toward Human.

Quick Overview for CD8 alpha antibody (FITC) (ABIN6655185)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: RPA-T8

Product Details anti-CD8 alpha Antibody (FITC)

Supplier Product No.: 200-302-m64

Supplier: Rockland

Purpose: CD8 Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD8 and chimpanzee.

Purification: Fluorescein conjugated CD8 Monoclonal Antibody was Protein G Purified from tissue culture supernatant and is directed against human CD8.

Sterility: Sterile filtered

Immunogen: Anti-CD8 Antibody (Monoclonal) was produced by repeated immunizations with CD8 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 2-8

Application Details

Application Notes: Optional[Neutralization_Dilution]: 10 μL/10^6 cells (0.25 μg)

Comment:

Anti-CD8 is tested for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8A

Background:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL

Background: CD8 is a cell surface glycoprotein expressing on thymocytes subsets and cytotoxic T cells and is either a homodimer of two alpha chains, or a heterodimer of one alpha and one beta chain. It acts as a coreceptor during T cell activation through the binding of MHC Class I molecules and is found on T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 associates with LCK with the help of a zinc clasp structure and down-regulates the production of major Th2-type cytokines. CD8 is also known to recruit pUL97 thereby inducing dissolution of the nuclear lamina and facilitating the nuclear export of viral capsids.

Gene Name: CD8

Gene ID: 925

NCBI Accession: NP_001139345

UniProt: P01732

Pathways: TCR Signaling