BIRC7 antibody

Catalog No. ABIN6655300

Supplier Product No.: 200-301-h90

The Mouse Monoclonal anti-BIRC7 antibody has been validated for WB, IF and FM. It is suitable to detect BIRC7 in samples from Human.

Quick Overview for BIRC7 antibody (ABIN6655300)

Target: BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BIRC7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 88C570

Product Details anti-BIRC7 Antibody

Supplier Product No.: 200-301-h90

Supplier: Rockland

Purpose: Livin Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Livin from human based on 100 % homology with the immunizing sequence.

Purification: Anti-Livin Antibody was purified by Protein G chromatography.

Immunogen: Livin Antibody was produced in mice prepared by repeated immunizations with recombinant human protein, Livin.

Isotype: IgG1 kappa

Application Details

Application Notes:

IF_Microscopy_Dilution: 2-5 μg/mL

Western_Blot_Dilution: 1-2 μg/mL

Comment:

Anti-Livin antibody is tested for use in IF/ICC and WB. Expect a band approximately 30kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for BIRC7

Target: BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))

Alternative Name: Livin

Background:

Synonyms: KIAP, LIVIN, MLIAP, RNF50, BIRC7, Baculoviral IAP repeat-containing protein 7, Kidney inhibitor of apoptosis protein, Melanoma inhibitor of apoptosis protein, ML-IAP, RING finger protein 50

Background: Anti-Livin BIRC7 KIAP antibody detects human Livin BIRC7 KIAP. The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently a novel human inhibitor of apoptosis protein (IAP) family member termed Livin or ML-IAP, which contains a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain, has been identified. Livin gene has two splicing variants that contain open reading frames of 298 and 280 amino acids and both contained a single copy of baculovirus IAP repeat (BIR) and RING domain. Both of the isoforms inhibit TNFa induced apoptosis in Jurkat cells. Livin expression is low in adult tissues. It is relatively expressed at a higher level in developmental tissues and in many cancer cells. Anti-Livin BIRC7 KIAP antibody is ideal for investigators invovled in apoptosis reasearch.

Gene Name: BIRC7

Gene ID: 79444

NCBI Accession: NP_071444

UniProt: Q96CA5