CD9 antibody (APC)

Catalog No. ABIN6655323

Supplier Product No.: 200-326-n70

This Mouse Monoclonal antibody specifically detects CD9 in FACS, WB and IHC. It exhibits reactivity toward Human.

Quick Overview for CD9 antibody (APC) (ABIN6655323)

Target: CD9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Clone: HI100

Product Details anti-CD9 Antibody (APC)

Supplier Product No.: 200-326-n70

Supplier: Rockland

Purpose: CD45RA Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD45RA.

Purification: Allophycocyanin conjugated CD45RA Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45RA.

Sterility: Sterile filtered

Immunogen: Anti-CD45RA Antibody (Monoclonal) was produced by repeated immunizations with CD45RA antigen.

Isotype: IgG2b kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

Western_Blot_Dilution: User Optimized

Comment:

Anti-CD45RA is tested for Flow Cytometry and is useful in Immunohistochemistry and Western Blot. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background:

Synonyms: CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD9, MIC3, TSPAN29, GIG2

Background: CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naive T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Gene Name: CD9

Gene ID: 928

NCBI Accession: NP_001760

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization