SLC7A11 antibody
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- Target See all SLC7A11 Antibodies
- SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SLC7A11 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)
- Purification
- This affinity purified antibody is directed against human xCT. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and orangutan, and 88% identity to mouse.
- Immunogen
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Immunogen: Anti-xCT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human xCT conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product SLC7A11 Primary Antibody
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- Application Notes
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Immunohistochemistry Dilution: 1:200
Application Note: Anti-xCT Antibody has been tested in Western Blot, ELISA, Immunofluorescence and Flow cytometry. Expect a band at 55.4 kDa in western blot using appropriate lysates. Positive control used NIH-3T3, Hela, HCT-116, A549, or PC3 whole cell lysates at 0.1-1.0 μg/mL for WB and PC3 with MeOH at 10 μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Immunoprecipitation Dilution: User Optimized
ELISA Dilution: 5.0 μg/mL
Flow Cytometry Dilution: 1:200
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 10 μg/mL
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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- Target
- SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))
- Alternative Name
- xCT (SLC7A11 Products)
- Synonyms
- SLC7A11 antibody, DKFZp468E122 antibody, CCBR1 antibody, xCT antibody, 9930009M05Rik antibody, AI451155 antibody, sut antibody, solute carrier family 7 member 11 antibody, solute carrier family 7, (cationic amino acid transporter, y+ system) member 11 antibody, solute carrier family 7 (cationic amino acid transporter, y+ system), member 11 antibody, SLC7A11 antibody, Slc7a11 antibody
- Background
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Synonyms: rabbit anti-xCT antibody, SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11
Background: xCT belongs to the amino acid-polyamine-organocation (APC) superfamily and L-type amino acid transporter (LAT) family. xCT gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. Increased expression of this gene results in neuronal cell death. xCT may be associated with kaposi sarcoma, dyscalculia, cystinuria, spondylolsis, and anemia of prematurity. Anti-xCT Antibody is useful for researchers interested in Cancer Research, Glucose/Energy Metabolism Research, and Cell Surface Protein Interaction Research.
Gene Name: SLC7A11
- Gene ID
- 23657
- NCBI Accession
- NP_055146
- UniProt
- Q9UPY5
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