SLC7A11 antibody

Catalog No. ABIN6655337

Supplier Product No.: 600-401-gu3s

This anti-SLC7A11 antibody is a Rabbit Polyclonal antibody detecting SLC7A11 in WB, IHC, ELISA, FACS, IF, IP and FM. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for SLC7A11 antibody (ABIN6655337)

Target: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC7A11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Product Details anti-SLC7A11 Antibody

Supplier Product No.: 600-401-gu3s

Supplier: Rockland

Purpose: xCT Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human xCT.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-xCT antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human xCT conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:200

Flow_Cytometry_Dilution: 1:200

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-xCT Antibody has been tested in Western Blot, ELISA, Immunofluorescence, and flow cytometry. Expect a band at 55.4 kDa in western blot using appropriate lysates. Positive control used HeLa, NIH-3T3, A549, HCT-116, or PC3 whole cell lysates at 0.1-1.0μg/mL for WB and PC3 with MeOH at 10μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-SLC7A11 antibody (ABIN6655337)

Tamiya, Urushihara, Shizuma, Ogawa, Nakai, Wakamatsu, Takenaka, Kakunaga: "SHARPIN Enhances Ferroptosis in Synovial Sarcoma Cells via NF-κB- and PRMT5-Mediated PGC1α Reduction." in: Cancers, Vol. 15, Issue 13, (2023) (PubMed).

Target Details for SLC7A11

Target: SLC7A11 (Solute Carrier Family 7, (Cationic Amino Acid Transporter, Y+ System) Member 11 (SLC7A11))

Alternative Name: xCT

Background:

Synonyms: rabbit anti-xCT antibody, SLC7A11, Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11

Background: xCT belongs to the amino acid-polyamine-organocation (APC) superfamily and L-type amino acid transporter (LAT) family. xCT gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. Increased expression of this gene results in neuronal cell death. xCT may be associated with kaposi sarcoma, dyscalculia, cystinuria, spondylolsis, and anemia of prematurity. Anti-xCT Antibody is useful for researchers interested in Cancer Research, Glucose/Energy Metabolism Research, and Cell Surface Protein Interaction Research.

Gene Name: SLC7A11

Gene ID: 23657

NCBI Accession: NP_055146

UniProt: Q9UPY5