DNAJB1 antibody

Catalog No. ABIN6655346

Supplier Product No.: 109-401-e57

This anti-DNAJB1 antibody is a Rabbit Polyclonal antibody detecting DNAJB1 in WB, IP and IF. Suitable for Human.

Quick Overview for DNAJB1 antibody (ABIN6655346)

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-DNAJB1 Antibody

Supplier Product No.: 109-401-e57

Supplier: Rockland

Purpose: HSP40 (HDJ1) Antibody

Cross-Reactivity (Details): This polyclonal antibody is specific for human Hsp40 protein.

Purification: Anti-Hsp40 Antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen: Hsp40 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against recombinant purified human hsp40.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Western_Blot_Dilution: 1: 1500-2,000

Comment:

Anti-Hsp40 Antibody has been tested in WB, IP, and IF. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Also detects recombinant Hsp40. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Alternative Name: Hsp40

Background:

Synonyms: Rabbit anti-Hsp40 Antibody, rabbit anti-heat shock protein 40 antibody, Mitochondrial protein import protein MAS5, Yeast dnaJ protein 1, YDJ1, MAS5, YNL064C, N2418, YNL2418C

Background: DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1, is a basic mammalian 40 kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1. Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli, an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK.

Gene Name: YDJ1

Gene ID: 3337

NCBI Accession: NP_006136

UniProt: P25685