S1PR4 antibody (N-Term)

Catalog No. ABIN6655347

Supplier Product No.: 600-401-i18

This Rabbit Polyclonal antibody specifically detects S1PR4 in WB, IF, IHC and FM. It exhibits reactivity toward Human.

Quick Overview for S1PR4 antibody (N-Term) (ABIN6655347)

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S1PR4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Product Details anti-S1PR4 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-i18

Supplier: Rockland

Purpose: Edg6 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Edg6 from human, mouse and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Edg6 Antibody was purified by affinity chromatography.

Immunogen: Edg6 Antibody was produced from whole rabbit serum prepared by repeated immunizations with the n-terminus amino acid portion of human Edg6 protein.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 3.0-5.0 μg/mL

Other: ICC: 5 μg/mL

Comment:

Anti-Edg6 is tested for use in WB, ICC/IF, IHC, and IHC-P. Expect a band approximately 35kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for S1PR4

Target: S1PR4 (Sphingosine-1-Phosphate Receptor 4 (S1PR4))

Alternative Name: Edg6

Background:

Synonyms: EDG6, Sphingosine 1-phosphate receptor 4, S1P receptor 4, S1P4, Endothelial differentiation G-protein coupled receptor 6, Sphingosine 1-phosphate receptor Edg-6, S1P receptor Edg-6

Background: Anti-Sphingolipid Receptor Edg6 S1P4 detects human and mouse Sphingolipid Receptor Edg6 S1P4. Edg6 is a recently cloned member of the endothelial differentiation gene (EDG) G protein-coupled receptor family. It is a receptor for the lysosphingolipid sphingosine 1-phosphate (S1P), which are structurally related lysophospholipid mediators. Binding of SPP to Edg6 activates Erk, leading to the activation of the transcription factor Elk1 and thus the mitogen-activated protein kinase (MAPK) signal transduction pathway. The protein also acts as a receptor for a lipid-derived ligand. Edg6 plays an essential role in lymphocyte cell signaling. The protein is specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Human Edg6 gene is intronless and is mapped to chromosome 19p13.3 at the D19S120 marker. Anti-Sphingolipid Receptor Edg6 S1P4 antibody is ideal for investigators involved in neurobiology.

Gene Name: S1PR4

UniProt: O95977