DDIT4 antibody (pThr23, pThr25)

Catalog No. ABIN6655385

Supplier Product No.: 600-401-x24

This anti-DDIT4 antibody is a Rabbit Polyclonal antibody detecting DDIT4 in WB and ELISA. Suitable for Human.

Quick Overview for DDIT4 antibody (pThr23, pThr25) (ABIN6655385)

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDIT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DDIT4 Antibody

Binding Specificity: pThr23, pThr25

Supplier Product No.: 600-401-x24

Supplier: Rockland

Purpose: REDD1 Phospho T23/25 Antibody

Cross-Reactivity (Details): Anti-REDD1 pT23/pT25 Antibody is directed against REDD1 protein phosphorylated at T23 and T25.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Thr23/25 REDD1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:5,000

Western_Blot_Dilution: 1:500

Comment:

Anti-Phospho-Thr23/25 REDD1 Antibody is tested for Western Blots and is specific for the ~34 kDa REDD1 phosphorylated at Thr23/25. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for DDIT4

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Alternative Name: REDD1

Background:

Synonyms: DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1

Background: REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli. REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress. It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A-DDB1-ROC1-b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 through the activity of GSK3b. REDD1 pT23/25 Antibody is ideal for researchers interested in cancer research.

Gene Name: DDIT4

Gene ID: 54541

NCBI Accession: NP_061931

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process