CD8 alpha antibody (FITC)

Catalog No. ABIN6655402

Supplier Product No.: 200-302-n61

The Mouse Monoclonal anti-CD8 alpha antibody is suitable to detect CD8 alpha in samples from Human. It has been validated for FACS and IHC.

Quick Overview for CD8 alpha antibody (FITC) (ABIN6655402)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: HIT8a

Product Details anti-CD8 alpha Antibody (FITC)

Supplier Product No.: 200-302-n61

Supplier: Rockland

Purpose: CD8a Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD8a, Marmoset, and chimpanzee.

Purification: Fluorescein conjugated CD8a Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD8a.

Sterility: Sterile filtered

Immunogen: Anti-CD8a Antibody (Monoclonal) was produced by repeated immunizations with CD8a antigen.

Isotype: IgG1 kappa

Labeling Ratio: 4-6

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

Comment:

Anti-CD8a has been tested in Flow Cytometry and is useful for Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Background:

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a

Background: CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Gene Name: CD8A

Gene ID: 925

NCBI Accession: NP_001139345

UniProt: P01732

Pathways: TCR Signaling