LSD1 antibody

Catalog No. ABIN6655416

Supplier Product No.: 200-401-w52

This Rabbit Polyclonal antibody specifically detects LSD1 in WB and ChIP. It exhibits reactivity toward Human.

Quick Overview for LSD1 antibody (ABIN6655416)

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LSD1 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Product Details anti-LSD1 Antibody

Supplier Product No.: 200-401-w52

Supplier: Rockland

Purpose: LSD1 Antibody

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-LSD1 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-LSD1 Antibody was produced in rabbits by repeated immunizations with a full length recombinant protein of human LSD1.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 3 μg per IP

Western_Blot_Dilution: 1:1,000

Comment:

Anti-LSD1 Antibody is tested for Chromatin Immunoprecipitation and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 110 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LSD1

Target: LSD1 (KDM1A) (Lysine (K)-Specific Demethylase 1A (KDM1A))

Alternative Name: LSD1

Background:

Synonyms: Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain-containing protein 2, AOF2, KDM1

Background: LSD1 (lysine specific demethylase 1) is a component of the histone demethylase complex that uses FAD as a prosthetic goup. LSD1 may have a dual effect on gene transcription. As it demethylates the mono- and dimethylated 'Lys-4' of histone H3, which are associated with transcriptional activation, LSD1 can act as a repressor of gene expression. However, LSD1 is also capable of demethylating 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to activation of androgen receptor target genes. LSD1 therefore is ideal for research in different processes such as Stem and Cancer Cell Biology, Embryonic Development, Epigenetics and Cell Differentiation and Proliferation.

Gene Name: KDM1A

Gene ID: 23028

NCBI Accession: NP_001009999

UniProt: O60341

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Negative Regulation of intrinsic apoptotic Signaling, Warburg Effect