CD11c antibody (APC)

Catalog No. ABIN6655425

Supplier Product No.: 200-326-n63

Product Details anti-CD11c Antibody (APC)

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11c antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 200-326-n63

Supplier: Rockland

Purpose: CD11c Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD11c, Baboon, Chimpanzee, Cynomolgus, and Rhesus.

Purification: Allophycocyanin conjugated CD11c Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD11c.

Sterility: Sterile filtered

Immunogen: Anti-CD11c Antibody (Monoclonal) was produced by repeated immunizations with CD11c antigen.

Clone: 3-9

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD11c is tested for Flow Cytometry and useful in Immunofluorescence and Immunoprecipitation. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD11c

Target: CD11c (ITGAX) (Integrin, alpha X (Complement Component 3 Receptor 4 Subunit) (ITGAX))

Alternative Name: CD11c (ITGAX Products)

Background:

Synonyms: Integrin alpha-X, CD11 antigen-like family member C, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150 95, CD11c

Background: The 3.9 antibody is widely used as a marker for CD11c expression on dendritic cells (DC), often in parallel with markers for CD11b, for identification of developmental stages and mature subsets of this cell type. CD11c is prominently expressed on tissue macrophages, and is also detected on activated neutrophils, granulocytes, some types of activated T cells and intestinal intraepithelial lymphocytes (IEL).L killing through its interactions with fibrinogen, CD54, and iC3b.

Gene Name: ITGAX

Gene ID: 3687

NCBI Accession: NP_000878

UniProt: P20702

Pathways: Complement System, Activated T Cell Proliferation, Integrin Complex