CD40 antibody (APC)

Catalog No. ABIN6655464

Supplier Product No.: 200-326-n67

This Mouse Monoclonal antibody specifically detects CD40 in FACS. It exhibits reactivity toward Human.

Quick Overview for CD40 antibody (APC) (ABIN6655464)

Target: CD40

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD40 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 5C3

Product Details anti-CD40 Antibody (APC)

Supplier Product No.: 200-326-n67

Supplier: Rockland

Purpose: CD40 Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD40.

Purification: Allophycocyanin conjugated CD40 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD40.

Sterility: Sterile filtered

Immunogen: Anti-CD40 Antibody (Monoclonal) was produced by repeated immunizations with CD40 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes: Optional[Neutralization_Dilution]: 5 uL/test/1x10e5 to 1x10e8 cells

Comment:

Anti-CD40 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background:

Synonyms: BP50, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

Background: CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Gene Name: CD40

Gene ID: 958

NCBI Accession: NP_001241

UniProt: P25942

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints