CDC6 antibody

Catalog No. ABIN6655469

Supplier Product No.: 200-301-p29

This anti-CDC6 antibody is a Mouse Monoclonal antibody detecting CDC6 in WB and ELISA. Suitable for Human.

Quick Overview for CDC6 antibody (ABIN6655469)

Target: CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: DCS-180

Product Details anti-CDC6 Antibody

Supplier Product No.: 200-301-p29

Supplier: Rockland

Purpose: CDC6 Antibody

Cross-Reactivity (Details): This product reacts with human.

Purification: Anti-CDC6 antibody was purified from mouse ascites by protein A chromatography.

Immunogen: CDC6 antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to human Cdc6.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Western_Blot_Dilution: 1-2 μg/mL

Comment:

Anti-CDC6 is tested for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62.7 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CDC6

Target: CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))

Alternative Name: CDC6

Background:

Synonyms: CDC18L, HsCdc18, p62(cdc6), CELL DIVISION CYCLE 18, S. POMBE, HOMOLOG-LIKE, CDC6 (cell division cycle 6, S. cerevisiae) homolog, CELL CYCLE CONTROLLER CDC6, CDC6-related protein, Cdc18-related protein

Background: In yeasts, Cdc6 (Saccharomyces cerevisiae) and Cdc18 (Schizosaccharomyces pombe) associate with the origin recognition complex (ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al. (1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells. This antibody is suitable for researchers interested in cancer research and Cell Cycle research.

Gene Name: CDC6

Gene ID: 990

NCBI Accession: NP_001245

UniProt: Q99741

Pathways: DNA Replication, Synthesis of DNA