CD27 antibody (FITC)

Catalog No. ABIN6655550

Supplier Product No.: 200-302-n65

This anti-CD27 antibody is a Mouse Monoclonal antibody detecting CD27 in FACS. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD27 antibody (FITC) (ABIN6655550)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD27 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: O323

Product Details anti-CD27 Antibody (FITC)

Supplier Product No.: 200-302-n65

Supplier: Rockland

Purpose: CD27 Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD27, Baboon, Cynomolgus, Rhesus, and Squirrel Monkey.

Purification: Fluorescein conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27.

Sterility: Sterile filtered

Immunogen: Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 4-6

Application Details

Application Notes: Optional[Neutralization_Dilution]: 5 µL/1x10e6 cells or 100μL of whole blood

Comment:

Anti-CD27 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

References for anti-CD27 antibody (FITC) (ABIN6655550)

Agematsu, Kobata, Sugita, Hirose, Schlossman, Morimoto: "Direct cellular communications between CD45R0 and CD45RA T cell subsets via CD27/CD70." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3627-35, (1995) (PubMed).

Hintzen, de Jong, Lens, van Lier: "CD27: marker and mediator of T-cell activation?" in: Immunology today, Vol. 15, Issue 7, pp. 307-11, (1994) (PubMed).

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background:

Synonyms: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7

Background: CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70 and plays an important role in costimulation of T cell activation, and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-kB and SAPK/JNK activation.

Gene Name: CD27

Gene ID: 939

NCBI Accession: NP_001233

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints