CES1 antibody

Catalog No. ABIN6655642

Supplier Product No.: 100-401-b14s

The Rabbit Polyclonal anti-CES1 antibody has been validated for WB, ELISA and IP. It is suitable to detect CES1 in samples from Human.

Quick Overview for CES1 antibody (ABIN6655642)

Target: CES1 (Carboxylesterase 1 (CES1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CES1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-CES1 Antibody

Supplier Product No.: 100-401-b14s

Supplier: Rockland

Purpose: Liver Carboxylesterase-1 Antibody

Cross-Reactivity (Details): The antibody detects recombinant hCE1 in cell lysates and native Liver Carboxylesterase-1 present in human tissue or fluids in various assays (ie.

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant human Liver Carboxylesterase-1.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1:100

ELISA_Dilution: 1:5,000 - 1:20,000

Western_Blot_Dilution: 1:2,500 - 1:5,000

Other: User Optimized

Comment:

This antibody has been tested for use in ELISA and western blotting and is specific for human CE1 (carboxylesterase).  Specific conditions for reactivity should be optimized by the end user.   Expect a band approximately 66 kDa in size corresponding CE1 by western blotting in the appropriate cell lysate or extract.  In western blot analysis endogenous protein present in human tissue or fluids, multiple bands of CE may appear corresponding to variable amount of glycosylation on the molecule.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 3 months

Target Details for CES1

Target: CES1 (Carboxylesterase 1 (CES1))

Alternative Name: CES1

Background:

Synonyms: rabbit anti-Liver Carboxylesterase-1 Antibody, Liver carboxylesterase 1, Acyl coenzyme A:cholesterol acyltransferase, ACAT, Monocyte/macrophage serine esterase, HMSE, Serine esterase 1, Brain carboxylesterase hBr1, Triacylglycerol hydrolase, Egasyn, Retinyl ester hydrolase, REH

Background: Carboxylesterases (CES) are ubiquitous enzymes responsible for the hydrolysis of endogenous substrates with ester, thioester, or amide bonds and of xenobiotics (effecting detoxification or drug metabolism). Drug half-life and bioavailability may be modulated by selective inhibition of these proteins. CES1 or hCE1 (also known as acyl coenzyme A:cholesterol acyltransferase, monocyte/macrophage serine esterase, HMSE, serine esterase 1, brain carboxylesterase, triacylglycerol hydrolase, egasyn and retinyl ester hydrolase) is responsible for hydrolysis of stored cholesterol esters in macrophage foam cells and release of free cholesterol for high density lipoprotein-mediated cholesterol efflux.

Gene Name: CES1

Gene ID: 1066, 68508965

UniProt: P23141

Pathways: Monocarboxylic Acid Catabolic Process