HIST1H4A antibody (Internal Region)

Catalog No. ABIN6655661

Supplier Product No.: 600-401-x46

Product Details anti-HIST1H4A Antibody

Target: HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H4A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Supplier Product No.: 600-401-x46

Supplier: Rockland

Purpose: Histone H4 pan Antibody

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-Histone H4 pan Antibody was purified by affinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Histone H4 pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the internal region of histone H4.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 2-5 μg per IP

ELISA_Dilution: 1:500

IF_Microscopy_Dilution: 1:1,000

Western_Blot_Dilution: 1:1,000

Other: Dot Blot: 1:10,000

Comment:

Anti-Histone H4 pan Antibody has been tested by Chromatin Immunoprecipitation, Western Blot, Immunofluorescence, and ELISA. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HIST1H4A

Target: HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

Alternative Name: HIST1H4A (HIST1H4A Products)

Background:

Synonyms: Histone H4, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4J, HIST1H4L, HIST2H4A, HIST2H4B, HIST4H4

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H4 pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Gene Name: HIST1H4A

Gene ID: 121504

NCBI Accession: NP_001029249

UniProt: P62805