APP antibody

Catalog No. ABIN6655723

Supplier Product No.: 200-401-e88

The Rabbit Polyclonal anti-APP antibody has been validated for WB, ELISA, IHC, IF, IP and FM. It is suitable to detect APP in samples from Human. There are 7+ publications available.

Quick Overview for APP antibody (ABIN6655723)

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Product Details anti-APP Antibody

Supplier Product No.: 200-401-e88

Supplier: Rockland

Purpose: Amyloid Oligomers (A11) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Amyloid Oligomers (A11) from Eukaryotes, Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Amyloid Oligomers (A11) Antibody was purified by Protein A chromatography.

Sterility: Sterile filtered

Immunogen: Amyloid Oligomers (A11) Antibody was produced from whole rabbit serum prepared by repeated immunizations with synthetic molecular mimic of soluble oligomers.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1:200

ELISA_Dilution: 0.1-10 μg/mL

Immunohistochemistry_Dilution: 1:1000-10,000

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:200

Comment:

Suggested Applications: Other
Anti-Amyloid Oligomers (A11) Antibody is tested for use in IP, IF microscopy, IHC, and WB. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-APP antibody (ABIN6655723)

Li, Guo, Chen, Chen, Fu, Chen: "Amyloid aggregates induced by the p53-R280T mutation lead to loss of p53 function in nasopharyngeal carcinoma." in: Cell death & disease, Vol. 15, Issue 1, pp. 35, (2024) (PubMed).

Liu, Lapcinski, Hlynialuk, Steuer, Loude, Shapiro, Kemper, Ashe: "Aβ∗56 is a stable oligomer that impairs memory function in mice." in: iScience, Vol. 27, Issue 3, pp. 109239, (2024) (PubMed).

Li, Li, Kang, Cui, Zheng, Wang, Han, Yu, Chang: "The divergent effects of astrocyte ceruloplasmin on learning and memory function in young and old mice." in: Cell death & disease, Vol. 13, Issue 11, pp. 1006, (2022) (PubMed).

Nnah, Lee, Wessling-Resnick: "Iron potentiates microglial interleukin-1β secretion induced by amyloid-β." in: Journal of neurochemistry, Vol. 154, Issue 2, pp. 177-189, (2020) (PubMed).

Fu, Jiang, Weng, Dick, Chang, Kassab: "Cerebrovascular miRNAs correlate with the clearance of Aβ through perivascular route in younger 3xTg-AD mice." in: Brain pathology (Zurich, Switzerland), Vol. 30, Issue 1, pp. 92-105, (2020) (PubMed).

Jeong, Kim, Chae, Lee, Yang, Kim, Kim, Lee, Lee, Choi, Yoon, Hwang: "Multifunctionalized Reduced Graphene Oxide Biosensors for Simultaneous Monitoring of Structural Changes in Amyloid-β 40." in: Sensors (Basel, Switzerland), Vol. 18, Issue 6, (2018) (PubMed).

Peng, Achariyar, Li, Liao, Mestre, Hitomi, Regan, Kasper, Peng, Ding, Benveniste, Nedergaard, Deane: "Suppression of glymphatic fluid transport in a mouse model of Alzheimer's disease." in: Neurobiology of disease, Vol. 93, pp. 215-25, (2018) (PubMed).

Target Details for APP

Target: APP (Amyloid beta (A4) Precursor Protein (APP))

Alternative Name: APP

Background:

Synonyms: Rabbit Anti-Amyloid Oligomer αβ Antibody, Rabbit Anti-Amyloid Oligomer alpha beta Antibody, Rabbit Anti-Amyloid Oligomers A11 Antibody, Amyloid beta A4 protein, Amyloid Oligomer AlphaBeta Antibody, APP Antibody, ABPP, APPI, Alzheimer disease amyloid protein, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II, A4, AD1

Background: Amyloid monomeric proteins can sometimes oligomerize into destructive amyloid fibrils. Amyloidogenic conformations of non-disease related proteins can be created by partial protein misfolding or denaturation. Many degenerative diseases are known to be related to the accumulation of misfolded proteins as amyloid fibres. These include the amyloid-β peptide plaques and tau neurofibrillary tangles in senile plaques of Alzheimer's symptomology, the deposition of α-synuclein in the Lewy bodies of Parkinson's disease, and accumulation of polyglutamine-containing aggregates in Huntington's disease. Anti-Amyloid Oligomers A11 Antibody is useful for researchers interested in Neuroscience research.

Gene Name: APP

Gene ID: 8666

NCBI Accession: NM_000484

UniProt: P05067

Pathways: Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour