PD-L1 antibody

Catalog No. ABIN6655838

Supplier Product No.: 600-101-gu8s

This anti-PD-L1 antibody is a Goat Polyclonal antibody detecting PD-L1 in WB, FACS, IHC, ELISA, IP, IF and FM. Suitable for Human.

Quick Overview for PD-L1 antibody (ABIN6655838)

Target: PD-L1 (CD274 (PD-L1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-PD-L1 Antibody

Supplier Product No.: 600-101-gu8s

Supplier: Rockland

Purpose: PDL1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human PDL1.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-PDL1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human PDL1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1.0 μg/mL

Comment:

Anti-PDL-1 Antibody has been tested in Western Blot, ELISA, and IF. Positive control whole cell lysates used MDA-MB-231 p/n (W09-001-GK6) and A549 p/n (W09-001-372) and positive recombinant protein control for western blotting. Expect a band at ~33.3 kDa in western blot using appropriate lysates. Immunofluorescence positive control cell line used was MDA-MB-231 p/n (W09-001-GK6). Although not tested, this antibody is likely functional in flow cytometry and immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PDL1

Background:

Synonyms: Goat anti-PDL1 antibody, Programmed cell death 1 ligand 1, CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1, B7 homolog 1, PD-L1, PDCD1 ligand 1, B7-H1, B7 homolog 1, PDL-1, CD274 Molecule antibody, MGC142294 antibody, PDL 1

Background: PDL1 belongs to the immunoglobulin superfamily and BTN/MOG family. PDL1 is involved in the costimulatory signal, essential for T-cell proliferation and production of IL-10 and IFNG, in an IL-2 dependent and a PDCD1 independent manner. It is up-regulated in T-and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation. PDL1 is up-regulated in B-cells activated by surface Ig cross-linking. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Anti-PDL-1 Antibody is useful for researches interested in cancer research, NF-kB pathway research, and the Immune system.

Gene Name: CD274

Gene ID: 29126

NCBI Accession: NP_001254635

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints