TLR5 antibody

Catalog No. ABIN6655865

Supplier Product No.: 200-301-i27

This anti-TLR5 antibody is a Mouse Monoclonal antibody detecting TLR5 in WB, IHC and FACS. Suitable for Human.

Quick Overview for TLR5 antibody (ABIN6655865)

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 85B152-5

Product Details anti-TLR5 Antibody

Supplier Product No.: 200-301-i27

Supplier: Rockland

Purpose: TLR5 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR5 from dog, human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR5 Antibody was purified by Protein G chromatography.

Immunogen: TLR5 Antibody was produced in mice prepared by repeated immunizations with a synthetic KLH-conjugated peptide corresponding to human TLR5.

Isotype: IgG2a kappa

Application Details

Application Notes:

Flow_Cytometry_Dilution: 1-3 μg/10^6 cells

Western_Blot_Dilution: 1-3 μg/mL

Comment:

Anti-TLR5 Antibody is tested for use in WB, Flow, Flow-CS, Flow-IC, IHC, and IHC-P. Expect a band approximately 97kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TLR5

Target: TLR5 (Toll-Like Receptor 5 (TLR5))

Alternative Name: TLR5

Background:

Synonyms: TIL3, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

Background: Anti-TLR5 Antibody detects human TLR5. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens. Anti-TLR5 Antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR5

Gene ID: 7100

NCBI Accession: NP_003259

UniProt: O60602

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades