KLF4 antibody (N-Term)

Catalog No. ABIN6655945

Supplier Product No.: 600-401-gu2s

The Rabbit Polyclonal anti-KLF4 antibody has been validated for WB, ELISA, IHC, IF, FACS, IP and FM. It is suitable to detect KLF4 in samples from Human.

Quick Overview for KLF4 antibody (N-Term) (ABIN6655945)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Product Details anti-KLF4 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-gu2s

Supplier: Rockland

Purpose: KLF4 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human KLF4.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-KLF4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Term portion of human KLF4 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-KLF4 Antibody has been tested in Western Blot, ELISA, and Immunofluorescence. Expect a band at ~54.7 kDa in western blot using appropriate lysates. Positive control whole cell lysates NCCIT and PC3 @ 1μg/mL for WB, and PC3 with PFA at 10μg/mL in IF. Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background:

Synonyms: rabbit anti-KLF4 Antibody, KLF-4 Antibody, Krueppel-like factor 4, Epithelial zinc finger protein EZF, EZF, Gut-enriched krueppel-like factor, GKLF, KLF 4

Background: KLF4 belong to the Kruppel like factors/Zinc fingers C2H2-type family. This gene encodes a protein that belongs to the Kruppel family of transcription factors. KLF4 can act both as activator and as repressor. It can bind the 5-CACCC-3 core sequence, the promoter region of its own gene, and can activate its own transcription. KLF4 regulates the expression of key transcription factors during embryonic development, playing an important role in maintaining embryonic stem cells, and in preventing their differentiation. This protein is required for normal development of the barrier function of skin postnatal, maintenance of the ocular surface, function in skeletal and kidney development, and thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. KLF4 may be associated with diseases such as Venous Hemangioma, Skin Squamous Cell Carcinoma, and pediatric lymphoma. Anti-KLF4 Antibody is useful for researchers interested in Peptide hormone metabolism, Stem Cell Differentiation Pathway Research, Wnt/Notch Pathway Research, and Cancer Research.

Gene Name: KLF4

Gene ID: 9314

NCBI Accession: NP_001300981

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance