EZH2 antibody (N-Term)

Catalog No. ABIN6656040

Supplier Product No.: 200-401-w53

This Rabbit Polyclonal antibody specifically detects EZH2 in WB, IHC, IF and ChIP. It exhibits reactivity toward Human.

Quick Overview for EZH2 antibody (N-Term) (ABIN6656040)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP)

Product Details anti-EZH2 Antibody

Binding Specificity: N-Term

Supplier Product No.: 200-401-w53

Supplier: Rockland

Purpose: EzH2 Antibody

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-EZH2 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-EZH2 Antibody was produced in rabbits by repeated immunizations containing an amino acid sequence from the N-terminus of mouse EZH2.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 2.5 μg per IP

Immunohistochemistry_Dilution: 1:100 - 1:500

Western_Blot_Dilution: 1:1,000

Comment:

Anti-EZH2 Antibody is tested for Chromatin Immunoprecipitation, Immunofluorescence, and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 85 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EzH2

Background:

Synonyms: Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2

Background: EZH2 is a histone-lysine methyltransferase which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression. It is a member of the polycomb group (PcG) family which form multimeric protein complexes and are involved in maintaining the transcriptional repressive state of genes over successive cell generations. The EZH2 activity is dependent on the association with other components of the PRC2 complex (EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7). EZH2 may play a role in the hematopoietic and internal nervous systems. Over-expression of EZH2 is observed during advanced stages of prostate cancer and breast cancer. Anti-EZH2 Antibody is ideal for research in Epigenetics, Gene Expression, Cell Biology and Cancer.

Gene Name: EZH2

Gene ID: 2146

NCBI Accession: NP_001190176

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation