SPTAN1 antibody

Catalog No. ABIN6656057

Supplier Product No.: 200-301-w92

This anti-SPTAN1 antibody is a Mouse Monoclonal antibody detecting SPTAN1 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for SPTAN1 antibody (ABIN6656057)

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTAN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3D7

Product Details anti-SPTAN1 Antibody

Supplier Product No.: 200-301-w92

Supplier: Rockland

Purpose: Alpha II Spectrin Antibody

Purification: Anti-Alpha II Spectrin Antibody was purified from tissue culture supernatant by protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Alpha II Spectrin Antibody was produced in mice by repeated immunizations with a recombinant construct containing the 7th, 8th, 9th repeats of human alpha II spectrin expressed in and purified from E. coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:500-1:2000

Western_Blot_Dilution: 1:2000

Other: ICC: 1:500-1:2000

Comment:

Anti-Alpha II Spectrin Antibody is tested for Western Blot, immunohistochemistry, and immunocytochemistry. Expect a band at approximately ~240 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SPTAN1

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Alternative Name: Alpha II Spectrin

Background:

Synonyms: Spectrin alpha chain non-erythrocytic 1, Fodrin alpha chain, Spectrin nonerythroid alpha subunit

Background: The spectrin family of cytoskeletal proteins is comprised of 2 alpha genes (α1 and α2) and five beta genes (β1-β5). Spectrins have been shown to function as scaffolding proteins in mechanical support of the plasma membrane as well as bind other membrane proteins and lipids. Defects in spectrin genes have been linked to some forms of hereditary spherocytosis, a type of auto-hemolytic anemia which is characterized by spherical red blood cells that are more prone to lysis. Alpha II Spectrin Antibody is ideal for researchers interested in cell cycle research.

Gene Name: SPTAN1

Gene ID: 6709

NCBI Accession: NP_001123910

UniProt: Q13813

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization