Doublecortin antibody

Catalog No. ABIN6656058

Supplier Product No.: 200-301-x04

This Mouse Monoclonal antibody specifically detects Doublecortin in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for Doublecortin antibody (ABIN6656058)

Target: Doublecortin (DCX)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 3E1

Product Details anti-Doublecortin Antibody

Supplier Product No.: 200-301-x04

Supplier: Rockland

Purpose: Doublecortin Antibody

Purification: Anti-Doublecortin Antibody was purified from tissue culture supernatant by protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Doublecortin Antibody was produced in mice by repeated immunizations with a full length recombinant human Doublecortin protein expressed in and purified from E. coli.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:50,000

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:5000

Other: ICC: 1:1000

Comment:

Anti-Doublecortin Antibody is tested for Western Blot, immunohistochemistry, and immunocytochemistry, it is specific for the ~35 & 45 kDa doublecortin protein. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: Doublecortin

Background:

Synonyms: Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DBCN, LISX

Background: Doublecortin, or DCX, is a microtubule associated protein that is expressed almost exclusively in very early neuronal development, making it an excellent marker for developing neuronal cells. Defects in the DCX gene lead to X-linked lissencephaly which is characterized by a lack of normal folds on the surface of the brain resulting in a smooth cerebral cortex caused by abnormal migration of neurons during development. Doublecortin Antibody is ideal for researchers interested in neuroscience research.

Gene Name: DCX

Gene ID: 1641

NCBI Accession: NP_000546

UniProt: O43602