CRYAA antibody

Catalog No. ABIN6656059

Supplier Product No.: 200-301-e84

The Mouse Monoclonal anti-CRYAA antibody has been validated for WB, ELISA and IF. It is suitable to detect CRYAA in samples from Human. There is 1 publication available.

Quick Overview for CRYAA antibody (ABIN6656059)

Target: CRYAA (Crystallin, alpha A (CRYAA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 1H3-B8

Product Details anti-CRYAA Antibody

Supplier Product No.: 200-301-e84

Supplier: Rockland

Purpose: Alpha A Crystallin Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Alpha A Crystallin from Human, Mouse, Cow, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Alpha A Crystallin Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Alpha Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha Crystallin protein.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:200

Western_Blot_Dilution: 1:2000

Comment:

Anti-Alpha A Crystallin Antibody has been tested in WB and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa protein corresponding to the molecular mass of α-crystallin on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CRYAA antibody (ABIN6656059)

Wei, Hao, Chen, Gan, Fan: "A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation." in: Experimental eye research, Vol. 226, pp. 109306, (2023) (PubMed).

Target Details for CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Alternative Name: Alpha A Crystallin

Background:

Synonyms: Heat shock protein beta4, Acry 1, CRYA1, CRYAA, HspB4, Alpha-crystallin A chain

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance. In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA. Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases.

Gene Name: CRYAA

Gene ID: 1409

NCBI Accession: NP_000385

UniProt: P02489

Pathways: M Phase