Rab4 antibody (C-Term)

Catalog No. ABIN6656358

Supplier Product No.: 100-401-g35

This Rabbit Polyclonal antibody specifically detects Rab4 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Rab4 antibody (C-Term) (ABIN6656358)

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rab4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Rab4 Antibody

Binding Specificity: C-Term

Supplier Product No.: 100-401-g35

Supplier: Rockland

Purpose: RAB4 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Rab4 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Rab4 Antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen: Rab4 Antibody was produced from whole rabbit serum prepared by repeated immunizations raised against a C-terminal region synthetic peptide from human Rab4.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000-2000

Comment:

Anti-Rab4 Antibody is tested by WB, IHC, and IF microscopy. Expect a band approximately ~26kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Rab4

Target: Rab4 (RAB4A) (RAB4A, Member RAS Oncogene Family (RAB4A))

Alternative Name: Rab4

Background:

Synonyms: Oncogene Rab4, Rab4A, Ras related protein Rab-4A

Background: Rab4 is a 25 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a sub-cellular compartment. Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper-variable COOH-terminal domains with a cysteine motif implicated in sub-cellular targeting. Post-translational modification of the cysteine motif with one or two geranylgeranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins. Each Rab shows a characteristic sub-cellular distribution. In particular, over-expression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. The presence of excessive Rab4 leads to the accumulation of tranferrin receptors in non-acidic, post-endosomal recycling vesicles considered an intermediate compartment between endosomes and plasma membranes. Rab4 also plays a role in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Mediating the association of Rab4 with transferring receptor-containing early endosomes takes place through the geranylgeranyl groups at its carboxyl-terminus. Membrane association is also cell cycle dependent, as phosphorylation at its c-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Gene Name: RAB4A

Gene ID: 5867

NCBI Accession: NP_004569

UniProt: P20338