MAP1LC3A antibody (N-Term)

Catalog No. ABIN6656617

Supplier Product No.: 200-401-h57

The Rabbit Polyclonal anti-MAP1LC3A antibody has been validated for WB and IHC. It is suitable to detect MAP1LC3A in samples from Human. There is 1 publication available.

Quick Overview for MAP1LC3A antibody (N-Term) (ABIN6656617)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MAP1LC3A Antibody

Binding Specificity: N-Term

Supplier Product No.: 200-401-h57

Supplier: Rockland

Purpose: ATG8 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-ATG8 from Opossum and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-ATG8 Antibody was purified by Protein G chromatography.

Immunogen: ATG8 Antibody was produced from whole rabbit serum prepared by repeated immunizations with at the n-terminus portion of human ATG8.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 5-10 μg/mL

Western_Blot_Dilution: 0.25-1 μg/mL

Comment:

Suggested Applications: IF
Anti-ATG8 antibody is tested by IHC-P and WB. Expect a band approximately 16 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-MAP1LC3A antibody (ABIN6656617)

Esposito, Falace, Wagner, Gal, Mei, Conti, Pisano, Aprile, Cerullo, De Fusco, Giovedì, Seibt, Magen, Polster, Eran, Stenton, Fiorillo, Ravid, Mayatepek, Hafner, Wortmann, Levanon, Marini, Mandel, Benfenati, Distelmaier, Fassio, Guerrini: "Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course." in: Brain : a journal of neurology, Vol. 142, Issue 12, pp. 3876-3891, (2020) (PubMed).

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: MAP1LC3A

Background:

Synonyms: Activating transcription factor 8

Background: Anti-ATG8 antibody detects human ATG8. MAP1A and MAP1B are microtubule-associated proteins made up of a heavy chain and multiple light chain components. One such light chain component associated with these proteins is ATG8. ATG8 (also called MAP1LC3A and APG8), an ~16KDa mammalian homolog of yeast Atg8, is a cytoplasmic protein that plays an essential role in the process and regulation of autophagy. ATG8 is an evolutionarily conserved protein that belongs to LC3 family. ATG8 interacts with various proteins in the cytoplasm including mammalian homologues of Atg4, Atg7, Atg3 and Atg12 and gets modified into LC3-I and LC3-II respectively. Both of these forms are suggested to be involved in the formation and intracellular trafficking of autophagosomes in mammalian cells. Anti-ATG8 antibody are ideal for investigators invovled in apoptosis research.

Gene Name: MAP1LC3A

Gene ID: 84557

NCBI Accession: NP_115903

UniProt: Q9H492

Pathways: Autophagy