DNAJB1 antibody

Catalog No. ABIN6656695

Supplier Product No.: 200-301-f56

The Mouse Monoclonal anti-DNAJB1 antibody has been validated for WB, ELISA, IHC, IP, IF and FM. It is suitable to detect DNAJB1 in samples from Human.

Quick Overview for DNAJB1 antibody (ABIN6656695)

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNAJB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: 3B9-E6

Product Details anti-DNAJB1 Antibody

Supplier Product No.: 200-301-f56

Supplier: Rockland

Purpose: HSP40 (HDJ1) Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp40 from Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-Hsp40 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Hsp40 Antibody was produced in mice by repeated immunizations raised against recombinant Protein Hsp40 (Hdj1).

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: 1:200

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:2000

Comment:

Anti-Hsp40 Antibody is tested for use in WB, IP, IHC, IF, and ELISA. Expect a band approximately ~40kDa protein corresponding to the molecular mass of Hsp40 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Alternative Name: Hsp40 (Hdj1)

Background:

Synonyms: DNAJ1, NDAJB1, HDJ1, Hsp40, HSPF1, DnaJ homolog subfamily B member 1, Dna J protein homolog 1, Heat shock 40 kDa protein 1, HSP40, heat shock protein 40, Human DnaJ protein 1, hDj-1

Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function. HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins. The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1. Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding.

Gene Name: DNAJB1

Gene ID: 3337

NCBI Accession: NP_006136

UniProt: P25685