GFAP antibody (AA 411-422)

Catalog No. ABIN6656791

Supplier Product No.: 200-301-w55

The Mouse Monoclonal anti-GFAP antibody has been validated for WB, IHC, FM and MA. It is suitable to detect GFAP in samples from Human. There is 1 publication available.

Quick Overview for GFAP antibody (AA 411-422) (ABIN6656791)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Clone: S206A-8

Product Details anti-GFAP Antibody

Binding Specificity: AA 411-422

Supplier Product No.: 200-301-w55

Supplier: Rockland

Purpose: GFAP Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-GFAP Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-GFAP Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Comment:

Anti-GFAP Antibody is tested for use in Western blot, Immunohistochemistry, and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFAP antibody (ABIN6656791)

Rodrigues-Amorim, Rivera-Baltanás, Del Carmen Vallejo-Curto, Rodriguez-Jamardo, de Las Heras, Barreiro-Villar, Blanco-Formoso, Fernández-Palleiro, Álvarez-Ariza, López, García-Caballero, Olivares, Spuch: "Plasma β-III tubulin, neurofilament light chain and glial fibrillary acidic protein are associated with neurodegeneration and progression in schizophrenia." in: Scientific reports, Vol. 10, Issue 1, pp. 14271, (2021) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background:

Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody

Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.

Gene Name: GFAP

Gene ID: 2670

UniProt: P14136