FTO antibody (N-Term)

Catalog No. ABIN6657007

Supplier Product No.: 200-301-d41

The Mouse Monoclonal anti-FTO antibody has been validated for WB and FM. It is suitable to detect FTO in samples from Human.

Quick Overview for FTO antibody (N-Term) (ABIN6657007)

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FTO antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Clone: 5-2H10

Product Details anti-FTO Antibody

Binding Specificity: N-Term

Supplier Product No.: 200-301-d41

Supplier: Rockland

Purpose: FTO Antibody

Cross-Reactivity (Details): Anti-FTO antibody is directed against human Fat mass and Obesity Related Protein.

Purification: Anti-FTO is a Protein G purified antibody from cell culture supernatant.

Immunogen: Anti-FTO Monoclonal Antibody was produced in mouse by repeated immunizations with FTO- Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-FTO (Mouse) Antibody has been tested in Western Blotting and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 58 kDa in size corresponding to FTO proteins in Western blots of rat testes lysate. This antibody has also been used for immunocytochemistry with neuronal progenitor cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FTO

Target: FTO (Fat Mass and Obesity-Associated (FTO))

Alternative Name: FTO

Background:

Synonyms: Alpha-ketoglutarate-dependent dioxygenase FTO, KIAA1752

Background: FTO Antibody detects human FTO which is the protein encoded by FTO gene that is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake. Deletion analysis of FTO gene in mice showed that FTO is functionally involved in the control of both energy intake and energy expenditure. Anti-FTO Antibody is ideal for investigators involved in Neuroscience and Cell Metabolism.

Gene Name: FTO

Gene ID: 79068

UniProt: Q9C0B1