CYP1A1 antibody

Catalog No. ABIN6657369

Supplier Product No.: 600-401-hb7

Product Details anti-CYP1A1 Antibody

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Supplier Product No.: 600-401-hb7

Supplier: Rockland

Purpose: Cytochrome P450 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human cytochrome p450.

Purification: This product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Cytochrome p450 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human cytochrome p450 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 5.0 μg/mL

Immunohistochemistry_Dilution: 1:100 - 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-Cytochrome p450 has been tested in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Expect a band at ~58kda in western blot using appropriate lysates. Positive controls used include an overexpressing lysate and PC-12 at 1μg/mL for WB, human kidney and/or lung for IHC, HepG2 for IF at 15 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CYP1A1

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Alternative Name: CYP1A1 (CYP1A1 Products)

Background:

Synonyms: Rabbit Anti-Cytochrome p450 Antibody, Rabbit Anti-CYP1A1 Antibody, CYPIA1, Cytochrome P450 form 6, Cytochrome P450-C, Cytochrome P450-P1

Background: Cytochrome p450 belongs to an enzyme superfamily that catalyzes many reactions such as the synthesis of cholesterol, lipids, steroids by oxidizing the compounds and plays a role in drug metabolism. The protein has been known to be stimulated by cigarette smoke. On-going studies link the cytochrome p450 protein with lung cancer. In the liver microsome, it is part of the NADPH-dependent electron transport pathway. The protein has been found expressed in the lung, lymphocytes and placenta. Anti-Cytochrome p450 antibody is useful for researchers interested in cancer research and enzyme research.

Gene Name: CYP1A1

Gene ID: 1543

NCBI Accession: NP_000490

UniProt: P04798

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha