CYP1A1 antibody (Internal Region)

Catalog No. ABIN870663

The Goat Polyclonal anti-CYP1A1 antibody (ABIN870663) specifically detects CYP1A1 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CYP1A1 antibody (Internal Region) (ABIN870663)

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This CYP1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-CYP1A1 Antibody

Binding Specificity: Internal Region

Purpose: cytochrome P450 1A1 (mouse)

Sequence: QDRKLDENAN VQLSD

Specificity: This antibody is not expected to cross-react with the similar P450 1A2

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QDRKLDENANVQLSD, from the internal region of the protein sequence according to NP_001129531.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 10 μg/mL.

Western Blot: Approx 55 kDa band observed in Human and Rat Lung lysates (calculated MW of 58.2 kDa according to Human NP_000490.1 and 59.4 kDa according to Rat NP_036672.). Primary incubation was 1 hour. Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CYP1A1

Target: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Alternative Name: cyp1a1

Background: Cyp1a1, cytochrome P450, family 1, subfamily A, polypeptide 1, AHH, AHRR, CP11, Cyp1a2, P450-1, CYPIA1, aromatic compound inducible, cytochrome P450 1A1, cytochrome P450 family 1 subfamily a polypeptide 2, cytochrome P450 subfamily I, polypeptide 1, cytoc

Molecular Weight: 59.2kDa

Gene ID: 13076, 24296

NCBI Accession: NP_001129531

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha