CYP1A1 antibody (Internal Region)
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- Target See all CYP1A1 Antibodies
- CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
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Binding Specificity
- Internal Region
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This CYP1A1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
- Purpose
- cytochrome P450 1A1 (mouse)
- Sequence
- QDRKLDENAN VQLSD
- Specificity
- This antibody is not expected to cross-react with the similar P450 1A2
- Cross-Reactivity
- Mouse, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-QDRKLDENANVQLSD, from the internal region of the protein sequence according to NP_001129531.1.
- Isotype
- IgG
- Top Product
- Discover our top product CYP1A1 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 10 μg/mL.
Western Blot: Approx 55 kDa band observed in Human and Rat Lung lysates (calculated MW of 58.2 kDa according to Human NP_000490.1 and 59.4 kDa according to Rat NP_036672.). Primary incubation was 1 hour. Recommended concentration: 0.1-0.3 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:64000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the ER and cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))
- Alternative Name
- cyp1a1 (CYP1A1 Products)
- Synonyms
- AHH antibody, AHRR antibody, CP11 antibody, CYP1 antibody, P1-450 antibody, P450-C antibody, P450DX antibody, Cyp1a2 antibody, P450-1 antibody, cyp1a1 antibody, CYP1A1 antibody, CYPIA1 antibody, Cyp45c antibody, Cypc45c antibody, P-450MC antibody, wu:fb63b04 antibody, zfCYP1A antibody, zgc:109747 antibody, ahh antibody, ahrr antibody, cp11 antibody, cyp1 antibody, cyp1a antibody, p1-450 antibody, p450-c antibody, p450dx antibody, cytochrome P450 family 1 subfamily A member 1 antibody, cytochrome P450 1A1 antibody, cytochrome P450, family 1, subfamily a, polypeptide 1 antibody, cytochrome P450, family 1, subfamily A, polypeptide 1 antibody, cytochrome P450 family 1 subfamily D polypeptide 1 antibody, cytochrome P4501A1 antibody, polycyclic hydrocarbon-inducible cytochrome P450c antibody, cytochrome P450, family 1, subfamily A antibody, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 antibody, cytochrome P450 family 1 subfamily A member 1 L homeolog antibody, CYP1A1 antibody, CpipJ_CPIJ010542 antibody, Cyp1a1 antibody, cyp1d1 antibody, LOC100328613 antibody, cyp1a antibody, LOC102129994 antibody, cyp1a1.L antibody
- Background
- Cyp1a1, cytochrome P450, family 1, subfamily A, polypeptide 1, AHH, AHRR, CP11, Cyp1a2, P450-1, CYPIA1, aromatic compound inducible, cytochrome P450 1A1, cytochrome P450 family 1 subfamily a polypeptide 2, cytochrome P450 subfamily I, polypeptide 1, cytoc
- Molecular Weight
- 59.2kDa
- Gene ID
- 13076, 24296
- NCBI Accession
- NP_001129531
- Pathways
- Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha
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