ATRIP antibody (pSer239)

Catalog No. ABIN6657444

Supplier Product No.: 600-401-w95

This Rabbit Polyclonal antibody specifically detects ATRIP in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ATRIP antibody (pSer239) (ABIN6657444)

Target: ATRIP (ATR Interacting Protein (ATRIP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATRIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ATRIP Antibody

Binding Specificity: pSer239

Supplier Product No.: 600-401-w95

Supplier: Rockland

Purpose: ATRIP Phospho S239 Antibody

Cross-Reactivity (Details): Anti-ATRIP pS239 Antibody is directed against human ATRIP protein phosphorylated at S239.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser239 ATRIP Antibody was produced from rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:5,000

Western_Blot_Dilution: 1:500

Comment:

Anti-ATRIP Phospho-Ser239 Antibody is tested for WB. Expect a band at approximately ~86 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user. The antibody also recognizes a band at ~32k.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ATRIP

Target: ATRIP (ATR Interacting Protein (ATRIP))

Alternative Name: ATRIP

Background:

Synonyms: ATM, Rad3-related-interacting protein, ATR-interacting protein

Background: ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at ser239. ATRIP pS239 Antibody is ideal for researchers interested in cancer research.

Gene Name: ATRIP

Gene ID: 84126

NCBI Accession: NP_001257951

UniProt: Q8WXE1