Annexin V antibody (Internal Region)

Catalog No. ABIN6657493

Supplier Product No.: 600-401-p51

The Rabbit Polyclonal anti-Annexin V antibody has been validated for WB and IHC. It is suitable to detect Annexin V in samples from Human.

Quick Overview for Annexin V antibody (Internal Region) (ABIN6657493)

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin V antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Annexin V Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-p51

Supplier: Rockland

Purpose: Annexin A5 Antibody

Cross-Reactivity (Details): Anti-Annexin A5 antibody is directed against human Annexin V protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Annexin A5 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Annexin V.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 0.5-1 μg/mL

Western_Blot_Dilution: 0.1-0.5 μg/mL

Other: Immunocytochemistry: 0.5-1 μg/mL

Comment:

Anti-Annexin A5 is tested for Immunohistochemistry-P, immunocytochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~35.9 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 200μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Annexin V

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: Annexin A5

Background:

Synonyms: Anchorin CII,Annexin V,Annexin-5,Calphobindin I,

Background: Annexin V also know as endonnexin II (ENX2), or placental protein 4 (PP4). Endonexin II is a member of the family of Ca(2+)-dependent phospholipid binding proteins, known as annexins. It bind to the phospholipids that are preferentially located on the cytosolic face of the plasma membrane. It has a relative molecular weight of about 35,000. The gene lies on mouse chromosome 3 in close linkage with the fibroblast growth factor 2 (basic) gene and is syntenic with other genes known to have orthologous counterparts on human chromosome 4q. The PP4 cDNA encoded a protein of 320 amino acid residues. A single mRNA, approximately 1.6 kb long, was found to be expressed in human cell lines and placenta. PP4 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. This antibody is suitable for researchers interested in cell signaling, apoptosis and neuroscience research.

Gene Name: ANXA5

Gene ID: 308

NCBI Accession: NP_001145

UniProt: P08758

Pathways: Apoptosis